全文获取类型
收费全文 | 25129篇 |
免费 | 506篇 |
国内免费 | 993篇 |
专业分类
林业 | 4507篇 |
农学 | 2496篇 |
基础科学 | 868篇 |
4196篇 | |
综合类 | 3584篇 |
农作物 | 2547篇 |
水产渔业 | 2153篇 |
畜牧兽医 | 2279篇 |
园艺 | 1511篇 |
植物保护 | 2487篇 |
出版年
2024年 | 18篇 |
2023年 | 132篇 |
2022年 | 315篇 |
2021年 | 432篇 |
2020年 | 402篇 |
2019年 | 370篇 |
2018年 | 3010篇 |
2017年 | 3066篇 |
2016年 | 1504篇 |
2015年 | 513篇 |
2014年 | 441篇 |
2013年 | 503篇 |
2012年 | 1418篇 |
2011年 | 2671篇 |
2010年 | 2680篇 |
2009年 | 1745篇 |
2008年 | 1788篇 |
2007年 | 2008篇 |
2006年 | 434篇 |
2005年 | 496篇 |
2004年 | 290篇 |
2003年 | 352篇 |
2002年 | 191篇 |
2001年 | 177篇 |
2000年 | 214篇 |
1999年 | 218篇 |
1998年 | 181篇 |
1997年 | 150篇 |
1996年 | 143篇 |
1995年 | 113篇 |
1994年 | 108篇 |
1993年 | 105篇 |
1992年 | 98篇 |
1991年 | 60篇 |
1990年 | 73篇 |
1989年 | 56篇 |
1988年 | 59篇 |
1987年 | 28篇 |
1986年 | 17篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1983年 | 9篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 4篇 |
1977年 | 4篇 |
1968年 | 4篇 |
1967年 | 1篇 |
1962年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 555 毫秒
71.
茭白主茎地上部养分积累和转运规律 总被引:1,自引:0,他引:1
以两个有代表性的茭白品种为试材,测定了植株主茎生育过程中地上各部位干、鲜质量的积累及叶片净光合速率的变化。结果表明在田间封行前,植株主茎叶和短缩茎的干、鲜质量持续缓慢增加;封行后至肉质茎快速膨大前,短缩茎的物质积累超过叶片和叶鞘;肉质茎快速膨大期间,干物质积累主要在其前期,同时短缩茎等其余部位干、鲜质量明显下降;之后肉质茎干、鲜质量增加趋缓,叶片、叶鞘质量仍持续下降,而短缩茎质量回升,进入新一轮物质积累期;主茎的总干物质量在封行后迅速提高,在肉质茎充分膨大后下降。功能叶片净光合速率在生育进程中总体呈持续缓慢下降的趋势,肉质茎快速膨大期间迅速大幅上升,紧接着又快速回落。 相似文献
72.
73.
74.
桂花在园林景观设计中的应用 总被引:2,自引:0,他引:2
对桂花植物造景的配置原则、园林应用进行概述和研究,提出因地制宜、层次错落、季相变化、背景烘托、民族传统的配置原则,并就园林应用上桂花的独立造景,桂花与其他植物的配置,桂花与山石、建筑物的配置作了分述。 相似文献
75.
AIM:The effective antisense sequences targeted VEGF mRNA with computer software would be screened and designed, and effect of them on growth K562 cells and protein expression of VEGF were studied with experiments.METHODS:Seven antisense sequences were selected and synthesized, which consisted of 18-20 deoxynucleotide acid and were modified with phosphorothioate, according to principle of low free energy of overall △G37 Overall. Cell growth was assayed by trypan blue dye exclusion assay and level of VEGF protien in the media was determined by ELISA.RESULTS:Six of seven sequences were capable of inhibing growth of K562 cells and downregulating the VEGF protein expression significantly, compared with Scrambed control group. It was found that there was a close correlation between low level of overall △G37 and antisense effectiveness (r=0.887,P<0.01).CONCLUSION:VEGF mRNA antisense oliogdeoxynucleotides, which were designed by computer software of RNAstructure, were able to inhibit growth of K562 and its protein expression. The VEGF mRNA may be new target attached by drugs. At same time, the computer aided design is useful methods to obtain the effective antisense. 相似文献
76.
AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P<0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P<0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner. 相似文献
77.
Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 相似文献
78.
Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献
79.
80.